LOCUS       MAN2A1-pGEn1            8661 bp    DNA     circular     20-SEP-2016
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_recomb     complement(3839..3867)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    7289..7830
                     /note="WPRE"
     misc_recomb     7251..7273
                     /label=attB2
                     /note="attB2 site"
                     /note="recognized as attB2"
     misc_feature    complement(7837..8051)
                     /note="BGH pA"
     misc_feature    complement(8139..8268)
                     /note="3'-ITR"
     misc_feature    2982..3056
                     /note="TCM signal sequence"
     misc_feature    3060..3083
                     /note="His tag"
     misc_feature    3084..3134
                     /note="AviTag"
     misc_feature    3135..3830
                     /note="Superfolder GFP"
     misc_feature    2976..2981
                     /note="Kozak seq"
     misc_feature    3868..3890
                     /note="TEV site"
     misc_feature    3891..7244
                     /note="OPTIMIZED MAN2A1 coding region"
     vector          join(7259..8661,1..3857)
                     /source="pGEn2-DEST"
                     /type="Destination Vector"
     clone           3858..7258
                     /source="MAN2A1 pDONR"
                     /type="Entry Clone"
     source          1..8661
                     /dnas_title="MAN2A1-pGEn1"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC
     3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC
     3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA
     3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG
     3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG
     3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC
     3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG
     3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC
     3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG
     3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA
     3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA
     3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG
     3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
     3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG
     3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT
     3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc GACCGGGGCC
     3901 ACCTGGACTA CCCCAGAAAC CCTAGAAGAG AGGGCAGCTT CCCCCAGGGC CAGCTGAGCA
     3961 TGCTGCAGGA AAAGATCGAC CACCTGGAAC GGCTGCTGGC CGAGAACAAC GAGATCATCA
     4021 GCAACATCCG GGACAGCGTG ATCAACCTGA GCGAGAGCGT GGAAGATGGC CCCAAGAGCA
     4081 GCCAGAGCAA CTTCTCTCAG GGCGCTGGCA GCCATCTGCT GCCTAGCCAG CTGTCTCTGA
     4141 GCGTGGACAC CGCCGATTGC CTGTTCGCCA GCCAGTCCGG CAGCCACAAT AGCGACGTGC
     4201 AGATGCTGGA CGTGTACAGC CTGATCAGCT TCGACAACCC CGACGGCGGC GTGTGGAAGC
     4261 AGGGCTTCGA TATCACCTAC GAGAGCAACG AGTGGGACAC CGAGCCCCTG CAGGTGTTCG
     4321 TGGTGCCTCA CAGCCACAAC GACCCCGGCT GGCTGAAAAC CTTCAACGAC TACTTCCGGG
     4381 ACAAGACCCA GTACATCTTC AACAACATGG TGCTGAAGCT GAAAGAGGAC AGCCGGCGGA
     4441 AGTTCATTTG GAGCGAGATC AGCTACCTGA GCAAGTGGTG GGACATCATC GACATCCAGA
     4501 AAAAGGACGC CGTGAAGTCC CTGATCGAGA ACGGCCAGCT GGAAATCGTG ACCGGCGGCT
     4561 GGGTCATGCC TGATGAGGCC ACCCCTCACT ACTTCGCCCT GATTGACCAG CTGATCGAGG
     4621 GCCACCAGTG GCTGGAAAAC AACATCGGCG TGAAGCCCAG AAGCGGCTGG GCCATCGATC
     4681 CTTTCGGCCA CAGCCCTACC ATGGCCTACC TGCTGAATAG AGCCGGCCTG AGCCACATGC
     4741 TGATCCAGAG GGTGCACTAT GCCGTGAAGA AGCACTTTGC CCTGCACAAG ACCCTGGAAT
     4801 TCTTCTGGCG GCAGAACTGG GACCTGGGCA GCGTGACCGA TATCCTGTGC CACATGATGC
     4861 CCTTCTACAG CTACGACATC CCCCACACCT GTGGCCCCGA CCCCAAGATC TGCTGTCAGT
     4921 TCGACTTCAA GCGGCTGCCA GGCGGCAGAT TCGGATGTCC TTGGGGCGTG CCCCCCGAGA
     4981 CAATCCACCC TGGCAATGTG CAGAGCCGGG CCAGAATGCT GCTGGACCAG TACCGGAAGA
     5041 AGTCCAAGCT GTTCCGGACC AAGGTGCTGC TGGCTCCCCT GGGCGACGAC TTCAGATACT
     5101 GCGAGTACAC CGAGTGGGAT CTGCAGTTCA AGAACTACCA GCAGCTGTTC GACTACATGA
     5161 ACTCCCAGAG CAAGTTCAAA GTGAAGATCC AGTTCGGCAC CCTGAGCGAT TTCTTCGACG
     5221 CCCTGGACAA GGCCGACGAG ACACAGAGAG ACAAGGGCCA GAGCATGTTC CCCGTGCTGA
     5281 GCGGCGACTT CTTCACCTAC GCCGACCGGG ACGACCACTA TTGGAGCGGC TACTTCACCA
     5341 GCCGGCCTTT CTACAAGCGG ATGGACCGGA TCATGGAAAG CCACCTGAGA GCCGCCGAGA
     5401 TCCTGTACTA TTTTGCCCTG AGACAGGCCC ACAAGTACAA GATCAACAAG TTCCTGAGCA
     5461 GCAGCCTGTA CACCGCCCTG ACCGAGGCCA GACGGAATCT GGGACTGTTC CAGCACCACG
     5521 ACGCCATCAC CGGCACCGCC AAAGATTGGG TGGTGGTGGA CTACGGCACC CGGCTGTTCC
     5581 ACTCCCTGAT GGTGCTGGAA AAGATTATCG GCAACAGCGC CTTCCTGCTG ATCCTGAAGG
     5641 ACAAGCTGAC CTACGACAGC TACAGCCCCG ACACCTTCCT GGAAATGGAC CTGAAGCAGA
     5701 AGTCCCAGGA CAGCCTGCCT CAGAAGAACA TCATCCGGCT GAGCGCCGAG CCCAGATACC
     5761 TGGTGGTGTA CAACCCCCTG GAACAGGACA GAATCAGCCT GGTGTCCGTG TACGTGTCCA
     5821 GCCCCACCGT GCAGGTGTTC AGCGCCTCTG GCAAGCCCGT GGAAGTGCAG GTGTCCGCCG
     5881 TGTGGGATAC CGCCAACACC ATCAGCGAGA CAGCCTACGA GATCTCCTTC CGGGCCCACA
     5941 TCCCTCCACT GGGCCTGAAG GTGTACAAGA TTCTGGAAAG CGCCAGCTCC AACAGCCACC
     6001 TGGCCGACTA CGTGCTGTAC AAGAACAAGG TGGAAGATAG CGGCATCTTC ACCATCAAGA
     6061 ACATGATCAA CACCGAGGAA GGCATCACCC TGGAAAATTC CTTCGTGCTG CTGAGATTCG
     6121 ACCAGACCGG CCTGATGAAG CAGATGATGA CCAAAGAGGA CGGCAAACAC CACGAAGTGA
     6181 ACGTGCAGTT CTCTTGGTAC GGCACCACAA TCAAGAGGGA CAAGAGCGGC GCCTACCTGT
     6241 TCCTGCCCGA CGGCAATGCC AAGCCCTACG TGTACACAAC CCCCCCATTC GTCAGAGTGA
     6301 CCCACGGCCG GATCTACAGC GAAGTGACCT GCTTCTTCGA CCACGTGACC CACAGAGTGC
     6361 GGCTGTACCA CATCCAGGGC ATCGAGGGAC AGTCTGTGGA AGTGTCCAAC ATCGTGGACA
     6421 TCCGGAAAGT GTACAACAGG GAAATCGCCA TGAAGATCAG CAGCGACATC AAGAGCCAGA
     6481 ACAGGTTCTA CACCGACCTG AACGGCTACC AGATCCAGCC CCGGATGACA CTGAGCAAGC
     6541 TGCCTCTGCA GGCCAACGTG TACCCCATGA CCACAATGGC CTACATCCAG GACGCCAAGC
     6601 ACCGGCTGAC ACTGCTGTCT GCTCAGTCCC TGGGCGTGTC CTCCCTGAAC AGCGGCCAGA
     6661 TCGAAGTGAT CATGGACAGA CGGCTGATGC AGGACGACAA CCGGGGACTG GAACAGGGCA
     6721 TCCAGGACAA CAAGATCACC GCCAACCTGT TCAGGATTCT GCTGGAAAAA CGCAGCGCCG
     6781 TGAATACCGA AGAGGAAAAG AAAAGCGTGT CCTACCCCAG CCTGCTGTCC CACATCACAT
     6841 CTTCCCTGAT GAACCACCCC GTGATCCCCA TGGCCAACAA GTTTAGCAGC CCAACCCTGG
     6901 AACTGCAGGG CGAGTTCAGC CCACTGCAGA GCAGCCTGCC CTGCGACATC CACCTCGTGA
     6961 ACCTGCGGAC CATCCAGAGC AAAGTGGGCA ACGGCCACTC CAACGAGGCC GCCCTGATCC
     7021 TGCACCGGAA GGGCTTTGAC TGCCGGTTCA GCAGCAAGGG CACCGGCCTG TTCTGTAGCA
     7081 CCACCCAGGG CAAGATCCTG GTGCAGAAAC TGCTGAACAA GTTCATCGTG GAAAGCCTGA
     7141 CCCCCAGCTC CCTGAGCCTG ATGCACTCTC CACCCGGCAC CCAGAACATC TCCGAGATCA
     7201 ATCTGAGCCC CATGGAAATC AGCACCTTCC GGATCCAGCT GCGCTAGGAC CCAGCTTTCT
     7261 TGTACAAAGT GGTTGATAGC TTGGATCCAA TCAACCTCTG GATTACAAAA TTTGTGAAAG
     7321 ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT
     7381 GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC
     7441 CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG
     7501 CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT
     7561 TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT
     7621 TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG
     7681 GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC
     7741 GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT
     7801 GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG AGATCTGCCT CGACTGTGCC TTCTAGTTGC
     7861 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
     7921 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
     7981 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
     8041 CATGCTGGGG ACTCGAGTTA AGGGCGAATT GCCGATTAGG ATCTTCCTAG AGCATGGCTA
     8101 CGTAGATAAG TAGCATGGCG GGTTAATCAT TAACTACAAG GAACCCCTAG TGATGGAGTT
     8161 GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG
     8221 ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC TTAATTAAGA
     8281 GCTCATGGCG CGCCTAGGCC TTGACGGCCT TCCGCCAATT CGCCCTATAG TGAGTCGTAT
     8341 TACGTCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC
     8401 CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC
     8461 CCGCACCGAA ACGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GAGCGCCCTG
     8521 TAGCGGCCAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT
     8581 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA
     8641 ATATTAACGC TTACAATTTA G
//

